View source for 8dej


==D. vulgaris type I-C Cascade bound to dsDNA target==
<StructureSection load='8dej' size='340' side='right'caption='[[8dej]], [[Resolution|resolution]] 2.86&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dej]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DEJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.86&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dej OCA], [https://pdbe.org/8dej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dej RCSB], [https://www.ebi.ac.uk/pdbsum/8dej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dej ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type I CRISPR-Cas systems employ multi-subunit Cascade effector complexes to target foreign nucleic acids for destruction. Here, we present structures of D. vulgaris type I-C Cascade at various stages of double-stranded (ds)DNA target capture, revealing mechanisms that underpin PAM recognition and Cascade allosteric activation. We uncover an interesting mechanism of non-target strand (NTS) DNA stabilization via stacking interactions with the "belly" subunits, securing the NTS in place. This "molecular seatbelt" mechanism facilitates efficient R-loop formation and prevents dsDNA reannealing. Additionally, we provide structural insights into how two anti-CRISPR (Acr) proteins utilize distinct strategies to achieve a shared mechanism of type I-C Cascade inhibition by blocking PAM scanning. These observations form a structural basis for directional R-loop formation and reveal how different Acr proteins have converged upon common molecular mechanisms to efficiently shut down CRISPR immunity.

Structural snapshots of R-loop formation by a type I-C CRISPR Cascade.,O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub , 2023 Feb 16. PMID:36805026<ref>PMID:36805026</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8dej" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Desulfovibrio vulgaris str. Hildenborough]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Bravo JPK]]
[[Category: Hibshman GN]]
[[Category: O'Brien RE]]
[[Category: Ramos D]]
[[Category: Taylor DW]]
[[Category: Wright JT]]