View source for 8dh4


==T7 RNA polymerase elongation complex with unnatural base dPa-DsTP pair==
<StructureSection load='8dh4' size='340' side='right'caption='[[8dh4]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dh4]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DH4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=S8U:[(2~{R},3~{S},5~{R})-5-(2-methanoylpyrrol-1-yl)-3-oxidanyl-oxolan-2-yl]methyl+dihydrogen+phosphate'>S8U</scene>, <scene name='pdbligand=S96:(7P)-3-{5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-ribofuranosyl}-7-(thiophen-2-yl)-3H-imidazo[4,5-b]pyridine'>S96</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dh4 OCA], [https://pdbe.org/8dh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dh4 RCSB], [https://www.ebi.ac.uk/pdbsum/8dh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dh4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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</StructureSection>
[[Category: Escherichia phage T7]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Oh J]]
[[Category: Wang D]]