View source for 8dk6


==Structure of hepatitis C virus envelope N-terminal truncated glycoprotein 2 (E2) (residues 456-713) from J6 genotype==
<StructureSection load='8dk6' size='340' side='right'caption='[[8dk6]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dk6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_C_virus_isolate_HC-J6 Hepatitis C virus isolate HC-J6] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DK6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dk6 OCA], [https://pdbe.org/8dk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dk6 RCSB], [https://www.ebi.ac.uk/pdbsum/8dk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dk6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2I6PIY1_9HEPC A0A2I6PIY1_9HEPC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hepatitis C virus (HCV) uses a hybrid entry mechanism. Current structural data suggest that upon exposure to low pH and Cluster of Differentiation 81 (CD81), the amino terminus of envelope glycoprotein E2 becomes ordered and releases an internal loop with two invariant aromatic residues into the host membrane. Here, we present the structure of an amino-terminally truncated E2 with the membrane binding loop in a bent conformation and the aromatic side chains sequestered. Comparison with three previously reported E2 structures with the same Fab indicates that this internal loop is flexible, and that local context influences the exposure of hydrophobic residues. Biochemical assays show that the amino-terminally truncated E2 lacks the baseline membrane-binding capacity of the E2 ectodomain. Thus, the amino terminal region is a critical determinant for both CD81 and membrane interaction. These results provide new insights into the HCV entry mechanism.

Regions of hepatitis C virus E2 required for membrane association.,Kumar A, Rohe TC, Elrod EJ, Khan AG, Dearborn AD, Kissinger R, Grakoui A, Marcotrigiano J Nat Commun. 2023 Jan 26;14(1):433. doi: 10.1038/s41467-023-36183-y. PMID:36702826<ref>PMID:36702826</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8dk6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hepatitis C virus isolate HC-J6]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Dearborn AD]]
[[Category: Elrod EJ]]
[[Category: Grakoui A]]
[[Category: Khan AG]]
[[Category: Kissinger R]]
[[Category: Kumar A]]
[[Category: Marcotrigiano J]]
[[Category: Rohe T]]