View source for 8dp8


==Crystal structure of the monomeric AvrM14-A Nudix hydrolase effector from Melampsora lini==
<StructureSection load='8dp8' size='340' side='right'caption='[[8dp8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dp8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Melampsora_lini Melampsora lini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DP8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.30001&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dp8 OCA], [https://pdbe.org/8dp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dp8 RCSB], [https://www.ebi.ac.uk/pdbsum/8dp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dp8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1B2CW89_MELLI A0A1B2CW89_MELLI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To infect plants, pathogenic fungi secrete small proteins called effectors. Here, we describe the catalytic activity and potential virulence function of the Nudix hydrolase effector AvrM14 from the flax rust fungus (Melampsora lini). We completed extensive in vitro assays to characterise the enzymatic activity of the AvrM14 effector. Additionally, we used in planta transient expression of wild-type and catalytically dead AvrM14 versions followed by biochemical assays, phenotypic analysis and RNA sequencing to unravel how the catalytic activity of AvrM14 impacts plant immunity. AvrM14 is an extremely selective enzyme capable of removing the protective 5' cap from mRNA transcripts in vitro. Homodimerisation of AvrM14 promoted biologically relevant mRNA cap cleavage in vitro and this activity was conserved in related effectors from other Melampsora spp. In planta expression of wild-type AvrM14, but not the catalytically dead version, suppressed immune-related reactive oxygen species production, altered the abundance of some circadian-rhythm-associated mRNA transcripts and reduced the hypersensitive cell-death response triggered by the flax disease resistance protein M1. To date, the decapping of host mRNA as a virulence strategy has not been described beyond viruses. Our results indicate that some fungal pathogens produce Nudix hydrolase effectors with in vitro mRNA-decapping activity capable of interfering with plant immunity.

A rust-fungus Nudix hydrolase effector decaps mRNA in vitro and interferes with plant immune pathways.,McCombe CL, Catanzariti AM, Greenwood JR, Desai AM, Outram MA, Yu DS, Ericsson DJ, Brenner SE, Dodds PN, Kobe B, Jones DA, Williams SJ New Phytol. 2023 Jul;239(1):222-239. doi: 10.1111/nph.18727. Epub 2023 Feb 2. PMID:36631975<ref>PMID:36631975</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8dp8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Melampsora lini]]
[[Category: Ericsson DJ]]
[[Category: McCombe CL]]
[[Category: Outram MA]]
[[Category: Williams SJ]]