View source for 8dut


==Duplex-G-quadruplex-duplex (DGD) class_1==
<StructureSection load='8dut' size='340' side='right'caption='[[8dut]], [[Resolution|resolution]] 7.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dut]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DUT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DUT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dut OCA], [https://pdbe.org/8dut PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dut RCSB], [https://www.ebi.ac.uk/pdbsum/8dut PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dut ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Genomic regions with high guanine content can fold into non-B form DNA four-stranded structures known as G-quadruplexes (G4s). Extensive in vivo investigations have revealed that promoter G4s are transcriptional regulators. Little structural information exists for these G4s embedded within duplexes, their presumed genomic environment. Here, we report the 7.4 A resolution structure and dynamics of a 28.5 kDa duplex-G4-duplex (DGD) model system using cryo-EM, molecular dynamics, and small-angle X-ray scattering (SAXS) studies. The DGD cryo-EM refined model features a 53 degrees bend induced by a stacked duplex-G4 interaction at the 5' G-tetrad interface with a persistently unstacked 3' duplex. The surrogate complement poly dT loop preferably stacks onto the 3' G-tetrad interface resulting in occlusion of both 5' and 3' tetrad interfaces. Structural analysis shows that the DGD model is quantifiably more druggable than the monomeric G4 structure alone and represents a new structural drug target. Our results illustrate how the integration of cryo-EM, MD, and SAXS can reveal complementary detailed static and dynamic structural information on DNA G4 systems.

Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 A resolution with cryo-EM.,Monsen RC, Chua EYD, Hopkins JB, Chaires JB, Trent JO Nucleic Acids Res. 2023 Feb 28;51(4):1943-1959. doi: 10.1093/nar/gkad014. PMID:36715343<ref>PMID:36715343</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8dut" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Chaires JB]]
[[Category: Chua EYD]]
[[Category: Hopkins JB]]
[[Category: Monsen RC]]
[[Category: Trent JO]]