View source for 8dxc


==[C:Hg2+/Ag+:C--pH 7.5] Metal-mediated DNA base pair in tensegrity triangle in Ag+ and Hg2+ solution==
<StructureSection load='8dxc' size='340' side='right'caption='[[8dxc]], [[Resolution|resolution]] 5.04&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dxc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DXC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.04&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dxc OCA], [https://pdbe.org/8dxc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dxc RCSB], [https://www.ebi.ac.uk/pdbsum/8dxc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dxc ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Metal-mediated DNA (mmDNA) presents a pathway toward engineering bioinorganic and electronic behavior into DNA devices. Many chemical and biophysical forces drive the programmable chelation of metals between pyrimidine base pairs. Here, we developed a crystallographic method using the three-dimensional (3D) DNA tensegrity triangle motif to capture single- and multi-metal binding modes across granular changes to environmental pH using anomalous scattering. Leveraging this programmable crystal, we determined 28 biomolecular structures to capture mmDNA reactions. We found that silver(I) binds with increasing occupancy in T-T and U-U pairs at elevated pH levels, and we exploited this to capture silver(I) and mercury(II) within the same base pair and to isolate the titration points for homo- and heterometal base pair modes. We additionally determined the structure of a C-C pair with both silver(I) and mercury(II). Finally, we extend our paradigm to capture cadmium(II) in T-T pairs together with mercury(II) at high pH. The precision self-assembly of heterobimetallic DNA chemistry at the sub-nanometer scale will enable atomistic design frameworks for more elaborate mmDNA-based nanodevices and nanotechnologies.

Heterobimetallic Base Pair Programming in Designer 3D DNA Crystals.,Lu B, Ohayon YP, Woloszyn K, Yang CF, Yoder JB, Rothschild LJ, Wind SJ, Hendrickson WA, Mao C, Seeman NC, Canary JW, Sha R, Vecchioni S J Am Chem Soc. 2023 Aug 2. doi: 10.1021/jacs.3c05478. PMID:37530628<ref>PMID:37530628</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8dxc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Lu B]]
[[Category: Ohayon YP]]
[[Category: Seeman NC]]
[[Category: Sha R]]
[[Category: Vecchioni S]]