View source for 8e2l


==Structure of Lates calcarifer Twinkle helicase with ATP and DNA==
<StructureSection load='8e2l' size='340' side='right'caption='[[8e2l]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8e2l]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Lates_calcarifer Lates calcarifer] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E2L FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.51&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e2l OCA], [https://pdbe.org/8e2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e2l RCSB], [https://www.ebi.ac.uk/pdbsum/8e2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e2l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A4W6C5C5_LATCA A0A4W6C5C5_LATCA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Twinkle is a mitochondrial replicative helicase which can self-load onto and unwind mitochondrial DNA. Nearly 60 mutations on Twinkle have been linked to human mitochondrial diseases. Using cryo-electron microscopy (cryo-EM) and high-speed atomic force microscopy (HS-AFM), we obtained the atomic-resolution structure of a vertebrate Twinkle homolog with DNA and captured in real-time how Twinkle is self-loaded onto DNA. Our data highlight the important role of the non-catalytic N-terminal domain of Twinkle. The N-terminal domain directly contacts the C-terminal helicase domain, and the contact interface is a hotspot for disease-related mutations. Mutations at the interface destabilize Twinkle hexamer and reduce helicase activity. With HS-AFM, we observed that a highly dynamic Twinkle domain, which is likely to be the N-terminal domain, can protrude approximately 5 nm to transiently capture nearby DNA and initialize Twinkle loading onto DNA. Moreover, structural analysis and subunit doping experiments suggest that Twinkle hydrolyzes ATP stochastically, which is distinct from related helicases from bacteriophages.

Structural and dynamic basis of DNA capture and translocation by mitochondrial Twinkle helicase.,Li Z, Kaur P, Lo CY, Chopra N, Smith J, Wang H, Gao Y Nucleic Acids Res. 2022 Nov 11;50(20):11965-11978. doi: 10.1093/nar/gkac1089. PMID:36400570<ref>PMID:36400570</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8e2l" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lates calcarifer]]
[[Category: Synthetic construct]]
[[Category: Gao Y]]
[[Category: Li Z]]