View source for 8e2o


==Leveraging the Structure of DNAJA1 to Discover Novel Pancreatic Cancer Therapies==
<StructureSection load='8e2o' size='340' side='right'caption='[[8e2o]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8e2o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E2O FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e2o OCA], [https://pdbe.org/8e2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e2o RCSB], [https://www.ebi.ac.uk/pdbsum/8e2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e2o ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/DNJA1_HUMAN DNJA1_HUMAN] Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
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== Publication Abstract from PubMed ==
Pancreatic cancer remains one of the deadliest forms of cancer with a 5-year survival rate of only 11%. Difficult diagnosis and limited treatment options are the major causes of the poor outcome for pancreatic cancer. The human protein DNAJA1 has been proposed as a potential therapeutic target for pancreatic cancer, but its cellular and biological functions remain unclear. Previous studies have suggested that DNAJA1's cellular activity may be dependent upon its protein binding partners. To further investigate this assertion, the first 107 amino acid structures of DNAJA1 were solved by NMR, which includes the classical J-domain and its associated linker region that is proposed to be vital to DNAJA1 functionality. The DNAJA1 NMR structure was then used to identify both protein and ligand binding sites and potential binding partners that may suggest the intracellular roles of DNAJA1. Virtual drug screenings followed by NMR and isothermal titration calorimetry identified 5 drug-like compounds that bind to two different sites on DNAJA1. A pull-down assay identified 8 potentially novel protein binding partners of DNAJA1. These proteins in conjunction with our previously published metabolomics study support a vital role for DNAJA1 in cellular oncogenesis and pancreatic cancer.

Leveraging the Structure of DNAJA1 to Discover Novel Potential Pancreatic Cancer Therapies.,Roth HE, De Lima Leite A, Palermo NY, Powers R Biomolecules. 2022 Sep 29;12(10). pii: biom12101391. doi: 10.3390/biom12101391. PMID:36291603<ref>PMID:36291603</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Powers R]]
[[Category: Roth HE]]