View source for 8exh


==Agrobacterium tumefaciens Tpilus==
<StructureSection load='8exh' size='340' side='right'caption='[[8exh]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8exh]] is a 40 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._C58 Agrobacterium fabrum str. C58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8EXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8EXH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=X3D:(14S,17R)-20-amino-17-hydroxy-11,17-dioxo-12,16,18-trioxa-17lambda~5~-phosphaicosan-14-yl+tetradecanoate'>X3D</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8exh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8exh OCA], [https://pdbe.org/8exh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8exh RCSB], [https://www.ebi.ac.uk/pdbsum/8exh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8exh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VIRB2_AGRFC VIRB2_AGRFC] VirB proteins are suggested to act at the bacterial surface and there play an important role in directing T-DNA transfer to plant cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Conjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient cell via an extracellular appendage known as the mating pilus. In bacteria, the conjugation machinery is encoded by plasmids or transposons and typically mediates the transfer of cognate mobile genetic elements. Much less is known about conjugation in archaea. Here, we determine atomic structures by cryo-electron microscopy of three conjugative pili, two from hyperthermophilic archaea (Aeropyrum pernix and Pyrobaculum calidifontis) and one encoded by the Ti plasmid of the bacterium Agrobacterium tumefaciens, and show that the archaeal pili are homologous to bacterial mating pili. However, the archaeal conjugation machinery, known as Ced, has been 'domesticated', that is, the genes for the conjugation machinery are encoded on the chromosome rather than on mobile genetic elements, and mediates the transfer of cellular DNA.

Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery.,Beltran LC, Cvirkaite-Krupovic V, Miller J, Wang F, Kreutzberger MAB, Patkowski JB, Costa TRD, Schouten S, Levental I, Conticello VP, Egelman EH, Krupovic M Nat Commun. 2023 Feb 7;14(1):666. doi: 10.1038/s41467-023-36349-8. PMID:36750723<ref>PMID:36750723</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8exh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Agrobacterium fabrum str. C58]]
[[Category: Large Structures]]
[[Category: Beltran LC]]
[[Category: Egelman EH]]