View source for 8ga3


==CLC-ec1 R230C/L249C/C85A at pH 4.5 100mM Cl Turn==
<StructureSection load='8ga3' size='340' side='right'caption='[[8ga3]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ga3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GA3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ga3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ga3 OCA], [https://pdbe.org/8ga3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ga3 RCSB], [https://www.ebi.ac.uk/pdbsum/8ga3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ga3 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CLCs are dimeric chloride channels and anion/proton exchangers that regulate processes such as muscle contraction and endo-lysosome acidification. Common gating controls their activity; its closure simultaneously silences both protomers, and its opening allows them to independently transport ions. Mutations affecting common gating in human CLCs cause dominant genetic disorders. The structural rearrangements underlying common gating are unknown. Here, using single-particle cryo-electron microscopy, we show that the prototypical Escherichia coli CLC-ec1 undergoes large-scale rearrangements in activating conditions. The slow, pH-dependent remodeling of the dimer interface leads to the concerted opening of the intracellular H(+) pathways and is required for transport. The more frequent formation of short water wires in the open H(+) pathway enables Cl(-) pore openings. Mutations at disease-causing sites favor CLC-ec1 activation and accelerate common gate opening in the human CLC-7 exchanger. We suggest that the pH activation mechanism of CLC-ec1 is related to the common gating of CLC-7.

Structural basis of pH-dependent activation in a CLC transporter.,Fortea E, Lee S, Chadda R, Argyros Y, Sandal P, Mahoney-Kruszka R, Ciftci HD, Falzone ME, Huysmans G, Robertson JL, Boudker O, Accardi A Nat Struct Mol Biol. 2024 Apr;31(4):644-656. doi: 10.1038/s41594-023-01210-5. , Epub 2024 Jan 26. PMID:38279055<ref>PMID:38279055</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ga3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Accardi A]]
[[Category: Argyos Y]]
[[Category: Boudker O]]
[[Category: Chadda R]]
[[Category: Ciftci D]]
[[Category: Fortea E]]
[[Category: Huysmans G]]
[[Category: Lee S]]
[[Category: Robertson JL]]