View source for 8ge0


==Crystal structure of JADE1 PZP domain in complex with Histone H3==
<StructureSection load='8ge0' size='340' side='right'caption='[[8ge0]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ge0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GE0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ge0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ge0 OCA], [https://pdbe.org/8ge0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ge0 RCSB], [https://www.ebi.ac.uk/pdbsum/8ge0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ge0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/JADE1_HUMAN JADE1_HUMAN] Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed:16387653, PubMed:19187766, PubMed:20129055, PubMed:24065767). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac), regulating DNA replication initiation, regulating DNA replication initiation (PubMed:20129055, PubMed:24065767). May also promote acetylation of nucleosomal histone H4 by KAT5 (PubMed:15502158). Promotes apoptosis (PubMed:16046545). May act as a renal tumor suppressor (PubMed:16046545). Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function (PubMed:22654112).<ref>PMID:15502158</ref> <ref>PMID:16046545</ref> <ref>PMID:16387653</ref> <ref>PMID:19187766</ref> <ref>PMID:20129055</ref> <ref>PMID:22654112</ref> <ref>PMID:24065767</ref> [https://www.uniprot.org/uniprot/H31_HUMAN H31_HUMAN] 
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== Publication Abstract from PubMed ==
JADE is a core subunit of the HBO1 acetyltransferase complex that regulates developmental and epigenetic programs and promotes gene transcription. Here we describe the mechanism by which JADE facilitates recruitment of the HBO1 complex to chromatin and mediates its enzymatic activity. Structural, genomic and complex assembly in vivo studies show that the PZP (PHD1-zinc-knuckle-PHD2) domain of JADE engages the nucleosome through binding to histone H3 and DNA and is necessary for the association with chromatin targets. Recognition of unmethylated H3K4 by PZP directs enzymatic activity of the complex toward histone H4 acetylation, whereas H3K4 hypermethylation alters histone substrate selectivity. We demonstrate that PZP contributes to leukemogenesis, augmenting transforming activity of the NUP98-JADE2 fusion. Our findings highlight biological consequences and the impact of the intact JADE subunit on genomic recruitment, enzymatic function and pathological activity of the HBO1 complex.

Guiding the HBO1 complex function through the JADE subunit.,Gaurav N, Kanai A, Lachance C, Cox KL, Liu J, Grzybowski AT, Saksouk N, Klein BJ, Komata Y, Asada S, Ruthenburg AJ, Poirier MG, Cote J, Yokoyama A, Kutateladze TG Nat Struct Mol Biol. 2024 Jul;31(7):1039-1049. doi: 10.1038/s41594-024-01245-2. , Epub 2024 Mar 6. PMID:38448574<ref>PMID:38448574</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ge0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Klein BJ]]
[[Category: Kutateladze TG]]
[[Category: Liu J]]