View source for 8gkh


==Structure of the Spizellomyces punctatus Fanzor (SpuFz) in complex with omega RNA and target DNA==
<StructureSection load='8gkh' size='340' side='right'caption='[[8gkh]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8gkh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei], [https://en.wikipedia.org/wiki/Spizellomyces_punctatus Spizellomyces punctatus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GKH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gkh OCA], [https://pdbe.org/8gkh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gkh RCSB], [https://www.ebi.ac.uk/pdbsum/8gkh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gkh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0F8NYV9_METMZ A0A0F8NYV9_METMZ] [https://www.uniprot.org/uniprot/A0A0L0H5U9_SPIPD A0A0L0H5U9_SPIPD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RNA-guided systems, which employ complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic elements, play a central role in biological processes in both prokaryotes and eukaryotes. For example, the prokaryotic CRISPR-Cas systems provide adaptive immunity for bacteria and archaea against foreign genetic elements. Cas effectors, like Cas9 and Cas12, perform guide RNA-dependent DNA cleavage(1). Although a few eukaryotic RNA-guided systems have been studied so far, such as RNA interference(2) and ribosomal RNA modification(3), it remains unclear whether eukaryotes have RNA-guided endonucleases. Recently, a new class of prokaryotic RNA-guided system (termed OMEGA) was reported(4,5). The OMEGA effector TnpB is the putative ancestor of Cas12 and has RNA-guided endonuclease activity(4,6). TnpB may also be the ancestor of the eukaryotic transposon-encoded Fanzor (Fz) proteins(4,7), raising the possibility that eukaryotes are also equipped with CRISPR-Cas/OMEGA-like programmable RNA-guided endonucleases. Here we report the biochemical characterization of Fz, showing that it is an RNA-guided DNA endonuclease. We also show that Fz can be reprogrammed for human genome engineering applications. Finally, we resolved the structure of Spizellomyces punctatus Fz (SpuFz) at 2.7A using cryogenic-electron microscopy, revealing the conservation of the core regions among Fz, TnpB and Cas12, despite diverse cognate RNA structures. Our results show that Fz is a eukaryotic OMEGA system, demonstrating that RNA-guided endonucleases are present in all three domains of life.

Fanzor is a eukaryotic programmable RNA-guided endonuclease.,Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, Desimone A, Macrae RK, Zhang F Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2. PMID:37380027<ref>PMID:37380027</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8gkh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanosarcina mazei]]
[[Category: Spizellomyces punctatus]]
[[Category: Synthetic construct]]
[[Category: Saito M]]
[[Category: Xu P]]
[[Category: Zhang F]]