View source for 8gu6


==Structure of the SbCas7-11-crRNA-NTR-Csx29 complex==
<StructureSection load='8gu6' size='340' side='right'caption='[[8gu6]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8gu6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GU6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gu6 OCA], [https://pdbe.org/8gu6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gu6 RCSB], [https://www.ebi.ac.uk/pdbsum/8gu6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gu6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0B0EKL4_9BACT A0A0B0EKL4_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The type III-E CRISPR-Cas systems are newly identified adaptive immune systems in prokaryotes that use a single Cas7-11 protein to specifically cleave target RNA. Cas7-11 could associate with Csx29, a putative caspase-like protein encoded by the gene frequently found in the type III-E loci, suggesting a functional linkage between the RNase and protease activities in type III-E systems. Here, we demonstrated that target RNA recognition would stimulate the proteolytic activity of Csx29, and protein Csx30 is the endogenous substrate. More interestingly, while the cognate target RNA recognition would activate Csx29, non-cognate target RNA with the complementary 3' anti-tag sequence inhibits the enzymatic activity. Csx30 could bind to the sigma factor RpoE, which may initiate the stress response after proteolytic cleavage. Combined with biochemical and structural studies, we have elucidated the mechanisms underlying the target RNA-guided proteolytic activity of Csx29. Our work will guide further developments leveraging this simple RNA targeting system for RNA and protein-related applications.

Target RNA-guided protease activity in type III-E CRISPR-Cas system.,Wang X, Yu G, Wen Y, An Q, Li X, Liao F, Lian C, Zhang K, Yin H, Wei Y, Deng Z, Zhang H Nucleic Acids Res. 2022 Dec 9;50(22):12913-12923. doi: 10.1093/nar/gkac1151. PMID:36484100<ref>PMID:36484100</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8gu6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Candidatus Scalindua brodae]]
[[Category: Large Structures]]
[[Category: Deng Z]]
[[Category: Wang X]]
[[Category: Yu G]]
[[Category: Zhang H]]