View source for 8gxs


==PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state==
<StructureSection load='8gxs' size='340' side='right'caption='[[8gxs]], [[Resolution|resolution]] 4.16&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8gxs]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa], [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GXS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.16&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gxs OCA], [https://pdbe.org/8gxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gxs RCSB], [https://www.ebi.ac.uk/pdbsum/8gxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gxs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2FA_HUMAN T2FA_HUMAN] TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.<ref>PMID:10428810</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RNA polymerase II-mediated eukaryotic transcription starts with the assembly of the preinitiation complex (PIC) on core promoters. The +1 nucleosome is well positioned about 40 base pairs downstream of the transcription start site (TSS) and is commonly known as a barrier of transcription. The +1 nucleosome-bound PIC-Mediator structures show that PIC-Mediator prefers binding to T40N nucleosome located 40 base pairs downstream of TSS and contacts T50N but not the T70N nucleosome. The nucleosome facilitates the organization of PIC-Mediator on the promoter by binding TFIIH subunit p52 and Mediator subunits MED19 and MED26 and may contribute to transcription initiation. PIC-Mediator exhibits multiple nucleosome-binding patterns, supporting a structural role of the +1 nucleosome in the coordination of PIC-Mediator assembly. Our study reveals the molecular mechanism of PIC-Mediator organization on chromatin and underscores the significance of the +1 nucleosome in regulating transcription initiation.

Structures of +1 nucleosome-bound PIC-Mediator complex.,Chen X, Wang X, Liu W, Ren Y, Qu X, Li J, Yin X, Xu Y Science. 2022 Oct 7;378(6615):62-68. doi: 10.1126/science.abn8131. Epub 2022 Oct , 6. PMID:36201575<ref>PMID:36201575</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8gxs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Synthetic construct]]
[[Category: Xenopus laevis]]
[[Category: Chen X]]
[[Category: Li J]]
[[Category: Liu W]]
[[Category: Qu X]]
[[Category: Ren Y]]
[[Category: Wang X]]
[[Category: Yin X]]