View source for 8gzo


==Crystal structure of collagen heterotrimer with K, D, E, R residuesC==
<StructureSection load='8gzo' size='340' side='right'caption='[[8gzo]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8gzo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chemical_production_metagenome Chemical production metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GZO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.23&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gzo OCA], [https://pdbe.org/8gzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gzo RCSB], [https://www.ebi.ac.uk/pdbsum/8gzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gzo ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Salt bridges are important factors in maintaining the stability of proteins, and their contribution to protein folding has received much attention. Although the interaction energies, or stabilizing contributions, of individual salt bridges have been measured in various proteins, a systematic assessment of various types of salt bridges in a relatively uniform environment is still a valuable analysis. Here, we used a collagen heterotrimer as a host-guest platform to construct 48 heterotrimers with the same charge pattern. A variety of salt bridges were formed between the oppositely charged residues Lys, Arg, Asp, and Glu. The melting temperature (T(m)) of the heterotrimers was measured with circular dichroism. The atomic structures of 10 salt bridges were shown in three x-ray crystals of heterotrimer. Molecular dynamics simulation based on the crystal structures indicated that strong, intermediate, and weak salt bridges have distinctive N-O distances. A linear regression model was used to predict the stability of heterotrimers with high accuracy (R(2) = 0.93). We developed an online database to help readers understand how a salt bridge stabilizes collagen. This work will help us better understand the stabilizing mechanism of salt bridges in collagen folding and provide a new strategy to design collagen heterotrimers.

Stability of collagen heterotrimer with same charge pattern and different charged residue identities.,Huang Y, Lan J, Wu C, Zhang R, Zheng H, Fan S, Xu F Biophys J. 2023 May 23:S0006-3495(23)00335-1. doi: 10.1016/j.bpj.2023.05.023. PMID:37226442<ref>PMID:37226442</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8gzo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Chemical production metagenome]]
[[Category: Large Structures]]
[[Category: Fan S]]