View source for 8ha2


==Crystal structure of voltage-gated sodium channel NavAb N49K/L176G mutant in calcium ion condition==
<StructureSection load='8ha2' size='340' side='right'caption='[[8ha2]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ha2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliarcobacter_butzleri Aliarcobacter butzleri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HA2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1N7:CHAPSO'>1N7</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PX4:1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PX4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ha2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ha2 OCA], [https://pdbe.org/8ha2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ha2 RCSB], [https://www.ebi.ac.uk/pdbsum/8ha2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ha2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8EVM5_ALIB4 A8EVM5_ALIB4] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Divalent cation block is observed in various tetrameric ion channels. For blocking, a divalent cation is thought to bind in the ion pathway of the channel, but such block has not yet been directly observed. So, the behaviour of these blocking divalent cations remains still uncertain. Here, we elucidated the mechanism of the divalent cation block by reproducing the blocking effect into NavAb, a well-studied tetrameric sodium channel. Our crystal structures of NavAb mutants show that the mutations increasing the hydrophilicity of the inner vestibule of the pore domain enable a divalent cation to stack on the ion pathway. Furthermore, non-equilibrium molecular dynamics simulation showed that the stacking calcium ion repel sodium ion at the bottom of the selectivity filter. These results suggest the primary process of the divalent cation block mechanism in tetrameric cation channels.

The structural basis of divalent cation block in a tetrameric prokaryotic sodium channel.,Irie K, Oda Y, Sumikama T, Oshima A, Fujiyoshi Y Nat Commun. 2023 Jul 15;14(1):4236. doi: 10.1038/s41467-023-39987-0. PMID:37454189<ref>PMID:37454189</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ha2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aliarcobacter butzleri]]
[[Category: Large Structures]]
[[Category: Irie K]]
[[Category: Oda Y]]