View source for 8har


==SAH-bound C-Methyltransferase Fur6 from Streptomyces sp. KO-3988==
<StructureSection load='8har' size='340' side='right'caption='[[8har]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8har]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._KO-3988 Streptomyces sp. KO-3988]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HAR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8har FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8har OCA], [https://pdbe.org/8har PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8har RCSB], [https://www.ebi.ac.uk/pdbsum/8har PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8har ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q2L6E4_STREO Q2L6E4_STREO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hybrid isoprenoid-polyketides, known as meroterpenoids, are a family of natural products that exhibit various bioactivities and are promising drug scaffolds. Despite the structural diversity of 1,3,6,8-tetrahydroxynaphthalene (THN)-derived meroterpenoids, such as furaquinocin, naphterpin, and furanonaphthoquinone, several biosynthetic genes for these compounds are conserved, suggesting a shared biosynthetic mechanism. However, the common biosynthetic mechanism and pathway-specific structural diversification mechanisms of these meroterpenoids are not yet fully understood. This study reveals the biosynthetic pathway for furaquinocin, demonstrating that it involves reductive deamination to form a key hydroquinone intermediate essential for subsequent reactions, including a unique cyclization step. We identified the mechanism of reductive deamination of the biosynthetic intermediate 8-amino-flaviolin through transient diazotization, leading to the formation of the hydroquinone intermediate 1,2,4,5,7-pentahydroxynaphthalene (PHN). Structural and computational studies confirmed that PHN is a key substrate for the subsequent methylation. We also showed that the hydroquinone intermediates are prerequisites for the subsequent pathway-specific reactions, including prenylation and novel intramolecular hydroalkoxylation of an alkene. This hydroalkoxylation reaction is notable in that a methyltransferase homolog catalyzes it in an S-adenosylmethionine-independent manner. Our findings provide a new model for furaquinocin biosynthesis, offering insights into the biosynthetic strategies for THN-derived meroterpenoids.

Biosynthesis of the tetrahydroxynaphthalene-derived meroterpenoid furaquinocin via reductive deamination and intramolecular hydroalkoxylation of an alkene.,Noguchi T, Zhao F, Moriwaki Y, Yamamoto H, Kudo K, Nagata R, Tomita T, Terada T, Shimizu K, Nishiyama M, Kuzuyama T Chem Sci. 2025 Mar 31;16(18):7912-7920. doi: 10.1039/d4sc08319a. eCollection 2025 , May 7. PMID:40191119<ref>PMID:40191119</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8har" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces sp. KO-3988]]
[[Category: Kuzuyama T]]
[[Category: Nagata R]]
[[Category: Noguchi T]]
[[Category: Tomita T]]