View source for 8hco


==Substrate-engaged TOM complex from yeast==
<StructureSection load='8hco' size='340' side='right'caption='[[8hco]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hco]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HCO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hco OCA], [https://pdbe.org/8hco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hco RCSB], [https://www.ebi.ac.uk/pdbsum/8hco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hco ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TOM40_YEAST TOM40_YEAST] Channel-forming protein essential for import of protein precursors into mitochondria.<ref>PMID:10427088</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Over half of mitochondrial proteins are imported from the cytosol via the pre-sequence pathway, controlled by the TOM complex in the outer membrane and the TIM23 complex in the inner membrane. The mechanisms through which proteins are translocated via the TOM and TIM23 complexes remain unclear. Here we report the assembly of the active TOM-TIM23 supercomplex of Saccharomyces cerevisiae with translocating polypeptide substrates. Electron cryo-microscopy analyses reveal that the polypeptide substrates pass the TOM complex through the center of a Tom40 subunit, interacting with a glutamine-rich region. Structural and biochemical analyses show that the TIM23 complex contains a heterotrimer of the subunits Tim23, Tim17 and Mgr2. The polypeptide substrates are shielded from lipids by Mgr2 and Tim17, which creates a translocation pathway characterized by a negatively charged entrance and a central hydrophobic region. These findings reveal an unexpected pre-sequence pathway through the TOM-TIM23 supercomplex spanning the double membranes of mitochondria.

Molecular pathway of mitochondrial preprotein import through the TOM-TIM23 supercomplex.,Zhou X, Yang Y, Wang G, Wang S, Sun D, Ou X, Lian Y, Li L Nat Struct Mol Biol. 2023 Dec;30(12):1996-2008. doi: 10.1038/s41594-023-01103-7. , Epub 2023 Sep 11. PMID:37696957<ref>PMID:37696957</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8hco" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Wang GP]]
[[Category: Wang SS]]
[[Category: Yang YQ]]
[[Category: Zhou XY]]