View source for 8hd2


==Crystal structure of SAM dependent methyltransferase encoded in type II fatty acid biosynthesis gene cluster from ladderane lipid producing anammox bacteria==
<StructureSection load='8hd2' size='340' side='right'caption='[[8hd2]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hd2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Brocadia_fulgida Candidatus Brocadia fulgida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HD2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hd2 OCA], [https://pdbe.org/8hd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hd2 RCSB], [https://www.ebi.ac.uk/pdbsum/8hd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hd2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0M2USF2_9BACT A0A0M2USF2_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ladderane lipids (found in the membranes of anaerobic ammonium-oxidizing [anammox] bacteria) have unique ladder-like hydrophobic groups, and their highly strained exotic structure has attracted the attention of scientists. Although enzymes encoded in type II fatty acid biosynthesis (FASII) gene clusters in anammox bacteria, such as S-adenosyl-l-methionine (SAM)-dependent enzymes, have been proposed to construct a ladder-like structure using a substrate connected to acyl carrier protein from anammox bacteria (AmxACP), no experimental evidence to support this hypothesis was reported to date. Here, we report the crystal structure of a SAM-dependent methyltransferase from anammox bacteria (AmxMT1) that has a substrate and active site pocket between a class I SAM methyltransferase-like core domain and an additional alpha-helix inserted into the core domain. Structural comparisons with homologous SAM-dependent C-methyltransferases in polyketide synthase, AmxACP pull-down assays, AmxACP/AmxMT1 complex structure predictions by AlphaFold, and a substrate docking simulation suggested that a small compound connected to AmxACP could be inserted into the pocket of AmxMT1, and then the enzyme transfers a methyl group from SAM to the substrate to produce branched lipids. Although the enzymes responsible for constructing the ladder-like structure remain unknown, our study, for the first time, supports the hypothesis that biosynthetic intermediates connected to AmxACP are processed by SAM-dependent enzymes, which are not typically involved in the FASII system, to produce the ladder-like structure of ladderane lipids in anammox bacteria.

Anammox Bacterial S-Adenosyl-l-Methionine Dependent Methyltransferase Crystal Structure and Its Interaction with Acyl Carrier Proteins.,Uegaki T, Takei T, Yamaguchi S, Fujiyama K, Sato Y, Hino T, Nagano S Int J Mol Sci. 2023 Jan 1;24(1):744. doi: 10.3390/ijms24010744. PMID:36614187<ref>PMID:36614187</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8hd2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Candidatus Brocadia fulgida]]
[[Category: Large Structures]]
[[Category: Hino T]]
[[Category: Nagano S]]
[[Category: Uegaki T]]