View source for 8hew


==Potato 14-3-3 St14f==
<StructureSection load='8hew' size='340' side='right'caption='[[8hew]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HEW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hew OCA], [https://pdbe.org/8hew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hew RCSB], [https://www.ebi.ac.uk/pdbsum/8hew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hew ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M1BI71_SOLTU M1BI71_SOLTU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Structure-based high-throughput screening of chemical compounds that target protein-protein interactions (PPIs) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At present, there are no examples of using high-throughput screening to identify chemicals that target plant transcriptional complexes, some of which are responsible for regulating multiple physiological functions. Florigen, a protein encoded by FLOWERING LOCUS T (FT), was initially identified as a molecule that promotes flowering and has since been shown to regulate flowering and other developmental phenomena such as tuber formation in potato (Solanum tuberosum). FT functions as a component of the florigen activation complex (FAC) with a 14-3-3 scaffold protein and FD, a bZIP transcription factor that activates downstream gene expression. Although 14-3-3 is an important component of FAC, little is known about the function of the 14-3-3 protein itself. Here, we report the results of a high-throughput in vitro fluorescence resonance energy transfer (FRET) screening of chemical libraries that enabled us to identify small molecules capable of inhibiting FAC formation. These molecules abrogate the in vitro interaction between the 14-3-3 protein and the OsFD1 peptide, a rice (Oryza sativa) FD, by directly binding to the 14-3-3 protein. Treatment with S4, a specific hit molecule, strongly inhibited FAC activity and flowering in duckweed, tuber formation in potato, and branching in rice in a dose-dependent manner. Our results demonstrate that the high-throughput screening approach based on the three-dimensional structure of PPIs is suitable in plants. In this study, we have proposed good candidate compounds for future modification to obtain inhibitors of florigen-dependent processes through inhibition of FAC formation.

Multifunctional chemical inhibitors of the florigen activation complex discovered by structure-based high-throughput screening.,Taoka KI, Kawahara I, Shinya S, Harada KI, Yamashita E, Shimatani Z, Furuita K, Muranaka T, Oyama T, Terada R, Nakagawa A, Fujiwara T, Tsuji H, Kojima C Plant J. 2022 Dec;112(6):1337-1349. doi: 10.1111/tpj.16008. Epub 2022 Nov 16. PMID:36288411<ref>PMID:36288411</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8hew" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Solanum tuberosum]]
[[Category: Fujiwara T]]
[[Category: Furuita K]]
[[Category: Harada K]]
[[Category: Kawahara I]]
[[Category: Kojima C]]
[[Category: Muranaka T]]
[[Category: Nakagawa A]]
[[Category: Shinya S]]
[[Category: Taoka K]]
[[Category: Tsuji H]]