View source for 8hs8


==a bacteria protein complex==
<StructureSection load='8hs8' size='340' side='right'caption='[[8hs8]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hs8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_parainfluenzae Haemophilus parainfluenzae] and [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HS8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hs8 OCA], [https://pdbe.org/8hs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hs8 RCSB], [https://www.ebi.ac.uk/pdbsum/8hs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hs8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0T7L299_NEIME A0A0T7L299_NEIME] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.[HAMAP-Rule:MF_01480]
__TOC__
</StructureSection>
[[Category: Haemophilus parainfluenzae]]
[[Category: Large Structures]]
[[Category: Neisseria meningitidis]]
[[Category: Li XZ]]
[[Category: Song GY]]
[[Category: Zhang H]]