View source for 8hvq


==Crystal structure of haloacid dehalogenase-like hydrolase family enzyme from Staphylococcus lugdunensis==
<StructureSection load='8hvq' size='340' side='right'caption='[[8hvq]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hvq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_lugdunensis Staphylococcus lugdunensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HVQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=OXM:OXAMIC+ACID'>OXM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hvq OCA], [https://pdbe.org/8hvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hvq RCSB], [https://www.ebi.ac.uk/pdbsum/8hvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hvq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A133Q9P0_STALU A0A133Q9P0_STALU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The haloacid dehalogenase superfamily implicated in bacterial pathogenesis comprises different enzymes having roles in many metabolic pathways. Staphylococcus lugdunensis, a Gram-positive bacterium, is an opportunistic human pathogen causing infections in the central nervous system, urinary tract, bones, peritoneum, systemic conditions and cutaneous infection. The haloacid dehalogenase superfamily proteins play a significant role in the pathogenicity of certain bacteria, facilitating invasion, survival, and proliferation within host cells. The genome of S. lugdunensis encodes more than ten proteins belonging to this superfamily. However, none of them have been characterized. The present work reports the characterization of one of the haloacid dehalogenase superfamily proteins (SLHAD1) from Staphylococcus lugdunensis. The functional analysis revealed that SLHAD1 is a metal-dependent acid phosphatase, which catalyzes the dephosphorylation of phosphorylated metabolites of cellular pathways, including glycolysis, gluconeogenesis, nucleotides, and thiamine metabolism. Based on the substrate specificity and genomic analysis, the physiological function of SLHAD1 in thiamine metabolism has been tentatively assigned. The crystal structure of SLHAD1, lacking 49 residues at the C-terminal, was determined at 1.7 A resolution with a homodimer in the asymmetric unit. It was observed that SLHAD1 exhibited time-dependent cleavage at a specific point, occurring through a self-initiated process. A combination of bioinformatics, biochemical, biophysical, and structural studies explored unique features of SLHAD1. Overall, the study revealed a detailed characterization of a critical enzyme of the human pathogen Staphylococcus lugdunensis, associated with several life-threatening infections.

Characterization of haloacid dehalogenase superfamily acid phosphatase from Staphylococcus lugdunensis.,Kaur H, Rode S, Kp S, Mahto JK, Alam MS, Gupta DN, Kar B, Singla J, Kumar P, Sharma AK Arch Biochem Biophys. 2024 Mar;753:109888. doi: 10.1016/j.abb.2024.109888. Epub , 2024 Jan 15. PMID:38232797<ref>PMID:38232797</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8hvq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus lugdunensis]]
[[Category: Kaur H]]
[[Category: Kumar P]]
[[Category: Mahto JK]]
[[Category: Sharma AK]]