View source for 8hx9


==Crystal structure of 4-amino-4-deoxychorismate synthase from Streptomyces venezuelae with chorismate==
<StructureSection load='8hx9' size='340' side='right'caption='[[8hx9]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hx9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HX9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ISJ:(3R,4R)-3-[(1-CARBOXYETHENYL)OXY]-4-HYDROXYCYCLOHEXA-1,5-DIENE-1-CARBOXYLIC+ACID'>ISJ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hx9 OCA], [https://pdbe.org/8hx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hx9 RCSB], [https://www.ebi.ac.uk/pdbsum/8hx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hx9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6L8Q5_STRVZ Q6L8Q5_STRVZ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
4-Amino-4-deoxychorismate synthase (ADCS), a chorismate-utilizing enzyme, is composed of two subunits: PabA and PabB. PabA is a glutamine amidotransferase that hydrolyzes glutamine into glutamate and ammonia. PabB is an aminodeoxychorismate synthase that converts chorismate to 4-amino-4-deoxychorismate (ADC) using the ammonia produced by PabA. ADCS functions under allosteric regulation between PabA and PabB. However, the allosteric mechanism remains unresolved because the structure of the PabA-PabB complex has not been determined. Here, the crystal structure and characterization of PapA from Streptomyces venezuelae (SvPapA), a bifunctional enzyme comprising the PabA and PabB domains, is reported. SvPapA forms a unique dimer in which PabA and PabB domains from different monomers complement each other and form an active structure. The chorismate-bound structure revealed that recognition of the C1 carboxyl group by Thr501 and Gly502 of the 498-PIKTG-502 motif in the PabB domain is essential for the catalytic Lys500 to reach the C2 atom, a reaction-initiation site. SvPapA demonstrated ADCS activity in the presence of Mg(2+) when glutamate or NH(+)(4) was used as the amino donor. The crystal structure indicated that the Mg(2+)-binding position changed depending on the binding of chorismate. In addition, significant structural changes were observed in the PabA domain depending on the presence or absence of chorismate. This study provides insights into the structural factors that are involved in the allosteric regulation of ADCS.

Structural basis for the allosteric pathway of 4-amino-4-deoxychorismate synthase.,Nakamichi Y, Kobayashi J, Toyoda K, Suda M, Hiraga K, Inui M, Watanabe M Acta Crystallogr D Struct Biol. 2023 Oct 1;79(Pt 10):895-908. doi: , 10.1107/S2059798323006320. Epub 2023 Sep 15. PMID:37712435<ref>PMID:37712435</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8hx9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces venezuelae]]
[[Category: Nakamichi Y]]
[[Category: Watanabe M]]