View source for 8imh


==Solution structure of the N terminal domain of MazE9 antitoxin (nMazE9) from Mycobacterium tuberculosis==
<StructureSection load='8imh' size='340' side='right'caption='[[8imh]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8imh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IMH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8imh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8imh OCA], [https://pdbe.org/8imh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8imh RCSB], [https://www.ebi.ac.uk/pdbsum/8imh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8imh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MAZE9_MYCTU MAZE9_MYCTU] Antitoxin component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and M.smegmatis neutralizes the effect of cognate toxin MazF9.<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:20876537</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The present study endeavors to decode the details of the transcriptional autoregulation effected by the MazE9 antitoxin of the Mycobacterium tuberculosis MazEF9 toxin-antitoxin system. Regulation of this bicistronic operon at the level of transcription is a critical biochemical process that is key for the organism's stress adaptation and virulence. Here, we have reported the solution structure of the DNA binding domain of MazE9 and scrutinized the thermodynamic and kinetic parameters operational in its interaction with the promoter/operator region, specific to the mazEF9 operon. A HADDOCK model of MazE9 bound to its operator DNA has been calculated based on the information on interacting residues obtained from these studies. The thermodynamics and kinetics of the interaction of MazE9 with the functionally related mazEF6 operon indicate that the potential for intracellular cross-regulation is unlikely. An interesting feature of MazE9 is the cis right harpoon over left harpoon trans conformational isomerization of proline residues in the intrinsically disordered C-terminal domain of this antitoxin.

Insights into the solution structure and transcriptional regulation of the MazE9 antitoxin in Mycobacterium tuberculosis.,Roy TB, Sarma SP Proteins. 2023 Sep 22. doi: 10.1002/prot.26589. PMID:37737533<ref>PMID:37737533</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8imh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Basu Roy T]]
[[Category: Sarma SP]]