View source for 8ini


==vaccinia H2 protein without the transmembrane region==
<StructureSection load='8ini' size='340' side='right'caption='[[8ini]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ini]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8INI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8INI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ini FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ini OCA], [https://pdbe.org/8ini PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ini RCSB], [https://www.ebi.ac.uk/pdbsum/8ini PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ini ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PG107_VACCW PG107_VACCW] Envelope protein part of the entry-fusion complex responsible for the virus membrane fusion with host cell membrane during virus entry. Also plays a role in cell-cell fusion (syncytium formation).<ref>PMID:15795260</ref> <ref>PMID:16339313</ref> <ref>PMID:18417576</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Vaccinia virus infection requires virus-cell membrane fusion to complete entry during endocytosis; however, it contains a large viral fusion protein complex of 11 viral proteins that share no structure or sequence homology to all the known viral fusion proteins, including type I, II, and III fusion proteins. It is thus very challenging to investigate how the vaccinia fusion complex works to trigger membrane fusion with host cells. In this study, we crystallized the ectodomain of vaccinia H2 protein, one component of the viral fusion complex. Furthermore, we performed a series of mutational, biochemical, and molecular analyses and identified two surface loops containing (170)LGYSG(174) and (125)RRGTGDAW(132) as the A28-binding region. We also showed that residues in the N-terminal helical region (amino acids 51-90) are also important for H2 function.

Structural and functional analyses of viral H2 protein of the vaccinia virus entry fusion complex.,Kao C-F, Liu C-Y, Hsieh C-L, Carillo KJD, Tzou D-LM, Wang H-C, Chang W J Virol. 2023 Dec 21;97(12):e0134323. doi: 10.1128/jvi.01343-23. Epub 2023 Nov , 17. PMID:37975688<ref>PMID:37975688</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ini" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vaccinia virus]]
[[Category: Chang W]]
[[Category: Ko TP]]
[[Category: Liu CY]]
[[Category: Wang HC]]