View source for 8iro


==trans-Zeatin bound state of Arabidopsis AZG1 at pH7.4==
<StructureSection load='8iro' size='340' side='right'caption='[[8iro]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8iro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IRO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZEA:(2E)-2-METHYL-4-(9H-PURIN-6-YLAMINO)BUT-2-EN-1-OL'>ZEA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8iro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8iro OCA], [https://pdbe.org/8iro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8iro RCSB], [https://www.ebi.ac.uk/pdbsum/8iro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8iro ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AZG1_ARATH AZG1_ARATH] Transports natural purines (adenine and guanine) as well as purine analogs. Confers sensitivity to 8-azaadenine and 8-azaguanine (8-azg).<ref>PMID:19121308</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytokinins are essential for plant growth and development, and their tissue distributions are regulated by transmembrane transport. Recent studies have revealed that members of the 'Aza-Guanine Resistant' (AZG) protein family from Arabidopsis thaliana can mediate cytokinin uptake in roots. Here we present 2.7 to 3.3 A cryo-electron microscopy structures of Arabidopsis AZG1 in the apo state and in complex with its substrates trans-zeatin (tZ), 6-benzyleaminopurine (6-BAP) or kinetin. AZG1 forms a homodimer and each subunit shares a similar topology and domain arrangement with the proteins of the nucleobase/ascorbate transporter (NAT) family. These structures, along with functional analyses, reveal the molecular basis for cytokinin recognition. Comparison of the AZG1 structures determined in inward-facing conformations and predicted by AlphaFold2 in the occluded conformation allowed us to propose that AZG1 may carry cytokinins across the membrane through an elevator mechanism.

Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1.,Xu L, Jia W, Tao X, Ye F, Zhang Y, Ding ZJ, Zheng SJ, Qiao S, Su N, Zhang Y, Wu S, Guo J Nat Plants. 2024 Jan;10(1):180-191. doi: 10.1038/s41477-023-01590-y. Epub 2024 , Jan 3. PMID:38172575<ref>PMID:38172575</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8iro" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Guo J]]
[[Category: Xu L]]