View source for 8iwk


==ABCG25 Wild Type purified with DDM plus CHS in ABA-bound state==
<StructureSection load='8iwk' size='340' side='right'caption='[[8iwk]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8iwk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IWK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8iwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8iwk OCA], [https://pdbe.org/8iwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8iwk RCSB], [https://www.ebi.ac.uk/pdbsum/8iwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8iwk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AB25G_ARATH AB25G_ARATH] High affinity abscisic acid (ABA) transporter that mediates the export of ABA, with a preference for (+)-ABA, through the plasma membrane, especially in vascular tissues (e.g. phloem companion cells), and is involved in the intercellular ABA signaling pathway (PubMed:20133881, PubMed:20935463, PubMed:24521878, PubMed:26517905). Together with ABCG31, export ABA from the endosperm to deliver it to the embryo via ABCG30 and ABCG40-mediated import to suppress radicle extension and subsequent embryonic growth (PubMed:26334616).<ref>PMID:20133881</ref> <ref>PMID:24521878</ref> <ref>PMID:26334616</ref> <ref>PMID:20935463</ref> <ref>PMID:26517905</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Abscisic acid (ABA) is a phytohormone essential to the regulation of numerous aspects of plant growth and development. The cellular level of ABA is critical to its signalling and is determined by its rate of biosynthesis, catabolism and the rates of ABA transport. ABCG25 in Arabidopsis thaliana has been identified to be an ABA exporter and play roles in regulating stomatal closure and seed germination. However, its ABA transport mechanism remains unknown. Here we report the structures of ABCG25 under different states using cryo-electron microscopy single particle analysis: the apo state and ABA-bound state of the wild-type ABCG25 and the ATP-bound state of the ATPase catalytic mutant. ABCG25 forms a homodimer. ABA binds to a cone-shaped, cytosolic-facing cavity formed in the middle of the transmembrane domains. Key residues in ABA binding are identified and verified by a cell-based ABA transport assay. ATP binding leads to closing of the nucleotide-binding domains of opposing monomers and conformational transitions of the transmembrane domains. Together, these results provide insights into the substrate recognition and transport mechanisms of ABCG25 in Arabidopsis, and facilitate our understanding of the ABA transport and signalling pathway in plants.

Structural basis for abscisic acid efflux mediated by ABCG25 in Arabidopsis thaliana.,Ying W, Liao L, Wei H, Gao Y, Liu X, Sun L Nat Plants. 2023 Oct;9(10):1697-1708. doi: 10.1038/s41477-023-01510-0. Epub 2023 , Sep 4. PMID:37666962<ref>PMID:37666962</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8iwk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Liao L]]
[[Category: Liu X]]
[[Category: Sun L]]
[[Category: Wei H]]
[[Category: Ying W]]