View source for 8ixi


==Crystal structure of macrolide phosphotransferase from Klebsiella pneumoniae==
<StructureSection load='8ixi' size='340' side='right'caption='[[8ixi]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ixi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IXI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.28&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ixi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ixi OCA], [https://pdbe.org/8ixi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ixi RCSB], [https://www.ebi.ac.uk/pdbsum/8ixi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ixi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D8L2F8_KLEPN D8L2F8_KLEPN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glutarate is a key monomer in polyester and polyamide production. The low efficiency of the current biosynthetic pathways hampers its production by microbial cell factories. Herein, through metabolic simulation, a lysine-overproducing E. coli strain Lys5 is engineered, achieving titer, yield, and productivity of 195.9 g/L, 0.67 g/g glucose, and 5.4 g/L.h, respectively. Subsequently, the pathway involving aromatic aldehyde synthase, monoamine oxidase, and aldehyde dehydrogenase (AMA pathway) is introduced into E. coli Lys5 to produce glutarate from glucose. To enhance the pathway's efficiency, rational mutagenesis on the aldehyde dehydrogenase is performed, resulting in the development of variant Mu5 with a 50-fold increase in catalytic efficiency. Finally, a glutarate tolerance gene cbpA is identified and genomically overexpressed to enhance glutarate productivity. With enzyme expression optimization, the glutarate titer, yield, and productivity of E. coli AMA06 reach 88.4 g/L, 0.42 g/g glucose, and 1.8 g/L.h, respectively. These findings hold implications for improving glutarate biosynthesis efficiency in microbial cell factories.

Systems engineering of Escherichia coli for high-level glutarate production from glucose.,Zhang Z, Chu R, Wei W, Song W, Ye C, Chen X, Wu J, Liu L, Gao C Nat Commun. 2024 Feb 3;15(1):1032. doi: 10.1038/s41467-024-45448-z. PMID:38310110<ref>PMID:38310110</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ixi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Klebsiella pneumoniae]]
[[Category: Large Structures]]
[[Category: Gao C]]
[[Category: Zhang ZL]]