View source for 8j52


==Crystal structure of Flavihumibacter petaseus GH31 alpha-galactosidase mutant D304A in complex with alpha-1,4-galactobiose==
<StructureSection load='8j52' size='340' side='right'caption='[[8j52]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8j52]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavihumibacter_petaseus_NBRC_106054 Flavihumibacter petaseus NBRC 106054]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J52 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j52 OCA], [https://pdbe.org/8j52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j52 RCSB], [https://www.ebi.ac.uk/pdbsum/8j52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j52 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0E9MUN5_9BACT A0A0E9MUN5_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glycoside hydrolase family 31 (GH31) contains alpha-glycoside hydrolases with different substrate specificities involved in various physiological functions. This family has recently been classified into 20 subfamilies using sequence similarity networks. An alpha-galactosidase from the gut bacterium Bacteroides salyersiae (BsGH31_19, which belongs to GH31 subfamily 19) was reported to have hydrolytic activity against the synthetic substrate p- nitrophenyl alpha-galactopyranoside, but its natural substrate remained unknown. BsGH31_19 shares low sequence identity (around 20%) with other reported GH31 alpha-galactosidases, PsGal31A from Pseudopedobacter saltans and human myogenesis-regulating glycosidase (MYORG), and was expected to have distinct specificity. Here, we characterized BsGH31_19 and its ortholog from a soil Bacteroidota bacterium, Flavihumibacter petaseus (FpGH31_19), and demonstrated that they showed high substrate specificity against alpha-(1--&gt;4)-linkages in alpha-(1--&gt;4)-galactobiose and globotriose [alpha-Gal-(1--&gt;4)-beta-Gal-(1--&gt;4)-Glc], unlike PsGal31A and MYORG. The crystallographic analyses of BsGH31_19 and FpGH31_19 showed that their overall structures resemble those of MYORG and form a dimer with an interface different from that of PsGal31A and MYORG dimers. The structures of FpGH31_19 complexed with d-galactose and alpha-(1--&gt;4)-galactobiose revealed that amino acid residues that recognize a galactose residue at subsite +1 are not conserved between FpGH31_19 and BsGH31_19. The tryptophan (Trp153) that recognizes galactose at subsite -1 is homologous to the tryptophan residues in MYORG and alpha-galactosidases belonging to GH27, GH36, and GH97, but not in the bacterial GH31 member PsGal31A. Our results provide structural insights into molecular diversity and evolutionary relationships in the GH31 alpha-galactosidase subfamilies and the other alpha-galactosidase families.

Structure-function analysis of bacterial GH31 alpha-galactosidases specific for alpha-(1--&gt;4)-galactobiose.,Ikegaya M, Park EY, Miyazaki T FEBS J. 2023 Jul 12. doi: 10.1111/febs.16904. PMID:37438884<ref>PMID:37438884</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8j52" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Flavihumibacter petaseus NBRC 106054]]
[[Category: Large Structures]]
[[Category: Ikegaya M]]
[[Category: Miyazaki T]]