View source for 8j68


==Crystal structure of the LOV1 R60K mutant of Klebsormidium nitens phototropin==
<StructureSection load='8j68' size='340' side='right'caption='[[8j68]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8j68]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsormidium_nitens Klebsormidium nitens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J68 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.845&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j68 OCA], [https://pdbe.org/8j68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j68 RCSB], [https://www.ebi.ac.uk/pdbsum/8j68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j68 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1Y1HNG4_KLENI A0A1Y1HNG4_KLENI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phototropin (Phot), a blue light-sensing LOV domain protein, mediates blue light responses and is evolutionarily conserved across the green lineage. Klebsormidium nitens, a green terrestrial alga, presents a valuable opportunity to study adaptive responses from aquatic to land habitat transitions. We determined the crystal structure of Klebsormidium nitens Phot LOV1 domain (KnLOV1) in the dark and engineered different mutations (R60K, Q122N, and D33N) to modulate the lifetime of the photorecovery cycle. We observed unusual, slow recovery kinetics in the wild-type KnLOV1 domain (tau = 41 +/- 3 min) compared to different mutants (R60K: tau = 2.0 +/- 0.1 min, Q122N: tau = 1.7 +/- 0.1 min, D33N: tau = 9.6 +/- 0.1 min). Crystal structures of wild-type KnLOV1 and mutants revealed subtle but critical changes near the protein chromophore that is responsible for modulating protein dark recovery time. Our findings shed light on the unique structural and biochemical characteristics of the newly studied KnLOV1 and its evolutionary importance for phototropin-mediated physiology.

Unusual photodynamic characteristics of the light-oxygen-voltage domain of phototropin linked to terrestrial adaptation of Klebsormidium nitens.,Sharma S, Gautam AK, Singh R, Gourinath S, Kateriya S FEBS J. 2024 Sep 29. doi: 10.1111/febs.17284. PMID:39344087<ref>PMID:39344087</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8j68" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Klebsormidium nitens]]
[[Category: Large Structures]]
[[Category: Gautam AK]]
[[Category: Gourinath S]]
[[Category: Kateriya S]]
[[Category: Sharma S]]