View source for 8jd8


==Crystal structure of Citrus limon Cu-Zn superoxide dismutase==
<StructureSection load='8jd8' size='340' side='right'caption='[[8jd8]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8jd8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrus_limon Citrus limon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JD8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JD8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jd8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jd8 OCA], [https://pdbe.org/8jd8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jd8 RCSB], [https://www.ebi.ac.uk/pdbsum/8jd8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jd8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q71S31_CITLI Q71S31_CITLI] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.[RuleBase:RU000393]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The copper-zinc superoxide dismutase (CuZnSOD) from lemon (SOD_CL) is active in an acidic environment and resists proteolytic degradation. The enzyme occurs as a dimer, which has an indirect effect on the enzyme activity as the monomer retains only approximately 35% of the activity. Here, the crystal structure of SOD_CL at 1.86 A resolution is reported that may explain this peculiarity. The crystal belonged to space group P2(1), with unit-cell parameters a = 61.11, b = 74.55, c = 61.69 A, beta = 106.86 degrees , and contained four molecules in the asymmetric unit. The overall structure of SOD_CL resembles that of CuZnSOD from plants. The structure of SOD_CL shows a unique arrangement of surface loop IV that connects the dimer interface and the active site, which is located away from the dimer-interface region. This arrangement allows direct interaction between the residues residing in the dimer interface and those in the active site. The arrangement also includes Leu62 and Gln164, which are conserved in cytoplasmic CuZnSOD. This supports the classification of SOD_CL as a cytoplasmic CuZnSOD despite sharing the highest amino-acid sequence homology with CuZnSODs from spinach and tomato, which are chloroplastic.

Direct relationship between dimeric form and activity in the acidic copper-zinc superoxide dismutase from lemon.,Utami RA, Yoshida H, Kartadinata LH, Abdillah VA, Faratilla CR, Retnoningrum DS, Ismaya WT Acta Crystallogr F Struct Biol Commun. 2023 Dec 1;79(Pt 12):301-307. doi: , 10.1107/S2053230X23010646. Epub 2023 Dec 18. PMID:38108885<ref>PMID:38108885</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8jd8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Citrus limon]]
[[Category: Large Structures]]
[[Category: Ismaya WT]]
[[Category: Retnoningrum DS]]
[[Category: Utami RA]]
[[Category: Yoshida H]]