View source for 8jqb


==Structure of Gabija GajA-GajB 4:4 Complex==
<StructureSection load='8jqb' size='340' side='right'caption='[[8jqb]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8jqb]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_VD045 Bacillus cereus VD045]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JQB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jqb OCA], [https://pdbe.org/8jqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jqb RCSB], [https://www.ebi.ac.uk/pdbsum/8jqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jqb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GAJA_BACC6 GAJA_BACC6] Component of antiviral defense system Gabija type I, composed of GajA and GajB. Expression of Gabija type I in B.subtilis (strain BEST7003) confers resistance to phages phi105, phi29, rho14, SpBeta and SBSphiC (PubMed:29371424). An endonuclease that nicks double-stranded DNA within the sequence 5'-TNNNCGGGNNA-3' in the absence of nucleotides (NTP, dNTP and NDPs), cleaving after C-1. Has no detected ATPase activity. Expression of Gabija type I in E.coli B (strain ATCC 11303) confers resistance to phage T7 (PubMed:33885789). It is thought that this enzyme is strongly suppressed during physiological growth (in E.coli total nucleotide concentration is over 8.7 mM in mid-log phase), but during viral replication, when nucleotides are rapidly consumed, it is de-suppressed and degrades target DNA (Probable).<ref>PMID:29371424</ref> <ref>PMID:33885789</ref> <ref>PMID:33885789</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus cereus VD045]]
[[Category: Large Structures]]
[[Category: Li J]]
[[Category: Wang L]]
[[Category: Wang Z]]