View source for 8jwd


==Histidine kinase QseE sensor domain of Escherichia coli O157:H7==
<StructureSection load='8jwd' size='340' side='right'caption='[[8jwd]], [[Resolution|resolution]] 1.33&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8jwd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JWD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.33&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jwd OCA], [https://pdbe.org/8jwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jwd RCSB], [https://www.ebi.ac.uk/pdbsum/8jwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jwd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/QSEE_ECO57 QSEE_ECO57] Member of the two-component regulatory system QseF/QseE involved in the regulation of virulence and metabolism in EHEC. Required for pedestal formation in host epithelial cells during infection. Autophosphorylates in response to epinephrine, sulfate or phosphate and then probably transfers its phosphate group to QseF.<ref>PMID:17220220</ref> <ref>PMID:19289831</ref> <ref>PMID:20056703</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacteria regulate virulence by using two-component systems (TCSs) composed of a histidine kinase (HK) and a response regulator (RR). TCSs respond to environmental signals and change gene-expression levels. The HK QseE and the RR QseF regulate the virulence of Enterobacteriaceae bacteria such as enterohemorrhagic Escherichia coli. The operon encoding QseE/QseF also contains a gene encoding an outer membrane lipoprotein, qseG. The protein product QseG interacts with QseE in the periplasmic space to control the activity of QseE and constitutes a unique QseE/F/G three-component system. However, the structural bases of their functions are unknown. Here, crystal structures of the periplasmic regions of QseE and QseG were determined with the help of AlphaFold models. The periplasmic region of QseE has a helix-bundle structure as found in some HKs. The QseG structure is composed of an N-terminal globular domain and a long C-terminal helix forming a coiled-coil-like structure that contributes to dimerization. Comparison of QseG structures obtained from several crystallization conditions shows that QseG has structural polymorphisms at the C-terminus of the coiled-coil structure, indicating that the C-terminus is flexible. The C-terminal flexibility is derived from conserved hydrophilic residues that reduce the hydrophobic interaction at the coiled-coil interface. Electrostatic surface analysis suggests that the C-terminal coiled-coil region can interact with QseE. The observed structural fluctuation of the C-terminus of QseG is probably important for interaction with QseE.

Crystal structures of QseE and QseG: elements of a three-component system from Escherichia coli.,Matsumoto K, Fukuda Y, Inoue T Acta Crystallogr F Struct Biol Commun. 2023 Nov 1;79(Pt 11):285-293. doi: , 10.1107/S2053230X23009123. Epub 2023 Oct 25. PMID:37877621<ref>PMID:37877621</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 8jwd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli O157:H7]]
[[Category: Large Structures]]
[[Category: Fukuda Y]]
[[Category: Inoue T]]
[[Category: Matsumoto K]]