View source for 8jxr


==Structure of nanobody-bound DRD1_LSD complex==
<StructureSection load='8jxr' size='340' side='right'caption='[[8jxr]], [[Resolution|resolution]] 3.57&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8jxr]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus], [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus], [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50] and [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JXR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.57&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jxr OCA], [https://pdbe.org/8jxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jxr RCSB], [https://www.ebi.ac.uk/pdbsum/8jxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jxr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DRD1_HUMAN DRD1_HUMAN] Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Understanding the kinetics of LSD in receptors and subsequent induced signaling is crucial for comprehending both the psychoactive and therapeutic effects of LSD. Despite extensive research on LSD's interactions with serotonin 2A and 2B receptors, its behavior on other targets, including dopamine receptors, has remained elusive. Here, we present cryo-EM structures of LSD/PF6142-bound dopamine D(1) receptor (DRD1)-legobody complexes, accompanied by a beta-arrestin-mimicking nanobody, NBA3, shedding light on the determinants of G protein coupling versus beta-arrestin coupling. Structural analysis unveils a distinctive binding mode of LSD in DRD1, particularly with the ergoline moiety oriented toward TM4. Kinetic investigations uncover an exceptionally rapid dissociation rate of LSD in DRD1, attributed to the flexibility of extracellular loop 2 (ECL2). Moreover, G protein can stabilize ECL2 conformation, leading to a significant slowdown in ligand's dissociation rate. These findings establish a solid foundation for further exploration of G protein-coupled receptor (GPCR) dynamics and their relevance to signal transduction.

Structural basis of psychedelic LSD recognition at dopamine D(1) receptor.,Fan L, Zhuang Y, Wu H, Li H, Xu Y, Wang Y, He L, Wang S, Chen Z, Cheng J, Xu HE, Wang S Neuron. 2024 Jul 25:S0896-6273(24)00494-X. doi: 10.1016/j.neuron.2024.07.003. PMID:39094559<ref>PMID:39094559</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8jxr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli O157:H7]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus subsp. aureus Mu50]]
[[Category: Streptococcus sp. 'group G']]
[[Category: Fan L]]
[[Category: Wang S]]
[[Category: Xu HE]]
[[Category: Xu Y]]
[[Category: Zhuang Y]]