View source for 8odo


==Structure of human guanylylated RTCB in complex with Archease==
<StructureSection load='8odo' size='340' side='right'caption='[[8odo]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8odo]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ODO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ODO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8odo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8odo OCA], [https://pdbe.org/8odo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8odo RCSB], [https://www.ebi.ac.uk/pdbsum/8odo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8odo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RTCB_HUMAN RTCB_HUMAN] Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.[HAMAP-Rule:MF_03144]<ref>PMID:21311021</ref> <ref>PMID:24870230</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pentameric human tRNA ligase complex. RNA ligation by the tRNA ligase complex requires GTP-dependent activation of RTCB. This active site guanylylation reaction relies on the activation factor Archease. The mechanistic interplay between both proteins has remained unknown. Here, we report a biochemical and structural analysis of the human RTCB-Archease complex in the pre- and post-activation state. Archease reaches into the active site of RTCB and promotes the formation of a covalent RTCB-GMP intermediate through coordination of GTP and metal ions. During the activation reaction, Archease prevents futile RNA substrate binding to RTCB. Moreover, monomer structures of Archease and RTCB reveal additional states within the RNA ligation mechanism. Taken together, we present structural snapshots along the reaction cycle of the human tRNA ligase.

Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease.,Gerber JL, Morales Guzman SI, Worf L, Hubbe P, Kopp J, Peschek J Nat Commun. 2024 Mar 16;15(1):2378. doi: 10.1038/s41467-024-46568-2. PMID:38493148<ref>PMID:38493148</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8odo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Gerber JL]]
[[Category: Kopp J]]
[[Category: Peschek J]]