View source for 8ofn


==Structure of the yellow fever virus (Asibi strain) dimeric envelope protein==
<StructureSection load='8ofn' size='340' side='right'caption='[[8ofn]], [[Resolution|resolution]] 3.48&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ofn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yellow_fever_virus Yellow fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OFN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.48&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ofn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ofn OCA], [https://pdbe.org/8ofn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ofn RCSB], [https://www.ebi.ac.uk/pdbsum/8ofn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ofn ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
All enveloped viruses enter cells by fusing their envelope with a target cell membrane while avoiding premature fusion with membranes of the producer cell-the latter being particularly important for viruses that bud at internal membranes. Flaviviruses bud in the endoplasmic reticulum, are transported through the TGN to reach the external milieu, and enter other cells via receptor-mediated endocytosis. The trigger for membrane fusion is the acidic environment of early endosomes, which has a similar pH to the TGN of the producer cell. The viral particles therefore become activated to react to mildly acidic pH only after their release into the neutral pH extracellular environment. Our study shows that for yellow fever virus (YFV), the mechanism of activation involves actively knocking out the fusion brake (protein pr) through a localized conformational change of the envelope protein upon exposure to the neutral pH external environment. Our study has important implications for understanding the molecular mechanism of flavivirus fusion activation in general and points to an alternative way of interfering with this process as an antiviral treatment.

New insight into flavivirus maturation from structure/function studies of the yellow fever virus envelope protein complex.,Crampon E, Covernton E, Vaney MC, Dellarole M, Sommer S, Sharma A, Haouz A, England P, Lepault J, Duquerroy S, Rey FA, Barba-Spaeth G mBio. 2023 Oct 31;14(5):e0070623. doi: 10.1128/mbio.00706-23. Epub 2023 Aug 21. PMID:37607061<ref>PMID:37607061</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Yellow fever virus]]
[[Category: Barba-Spaeth G]]
[[Category: Covernton E]]
[[Category: Rey FA]]
[[Category: Vaney MC]]