View source for 8ofr


==Human adenovirus type 25 fiber-knob protein complexed with sialic acid==
<StructureSection load='8ofr' size='340' side='right'caption='[[8ofr]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ofr]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_25 Human adenovirus 25]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OFR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SLB:5-N-ACETYL-BETA-D-NEURAMINIC+ACID'>SLB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ofr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ofr OCA], [https://pdbe.org/8ofr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ofr RCSB], [https://www.ebi.ac.uk/pdbsum/8ofr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ofr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M0QUM1_9ADEN M0QUM1_9ADEN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human adenoviruses (HAdV) are widespread pathogens causing usually mild infections. The Species D (HAdV-D) cause gastrointestinal tract infections and epidemic keratoconjunctivitis (EKC). Despite being significant pathogens, knowledge around HAdV-D mechanism of cell infection is lacking. Sialic acid (SA) usage has been proposed as a cell infection mechanism for EKC causing HAdV-D. Here we highlight an important role for SA engagement by many HAdV-D. We provide apo state crystal structures of 7 previously undetermined HAdV-D fiber-knob proteins, and structures of HAdV-D25, D29, D30 and D53 fiber-knob proteins in complex with SA. Biologically, we demonstrate that removal of cell surface SA reduced infectivity of HAdV-C5 vectors pseudotyped with HAdV-D fiber-knob proteins, whilst engagement of the classical HAdV receptor CAR was variable. Our data indicates variable usage of SA and CAR across HAdV-D. Better defining these interactions will enable improved development of antivirals and engineering of the viruses into refined therapeutic vectors.

Broad sialic acid usage amongst species D human adenovirus.,Mundy RM, Baker AT, Bates EA, Cunliffe TG, Teijeira-Crespo A, Moses E, Rizkallah PJ, Parker AL Npj Viruses. 2023;1(1):1. doi: 10.1038/s44298-023-00001-5. Epub 2023 Sep 26. PMID:38665237<ref>PMID:38665237</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ofr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human adenovirus 25]]
[[Category: Large Structures]]
[[Category: Baker AT]]
[[Category: Mundy RM]]
[[Category: Parker AL]]
[[Category: Rizkallah PJ]]