View source for 8ojj


==Cryo-EM structure of the DnaD-NTD tetramer==
<StructureSection load='8ojj' size='340' side='right'caption='[[8ojj]], [[Resolution|resolution]] 5.47&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ojj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OJJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.47&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ojj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ojj OCA], [https://pdbe.org/8ojj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ojj RCSB], [https://www.ebi.ac.uk/pdbsum/8ojj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ojj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DNAD_BACSU DNAD_BACSU] Probable component of primosome involved in the initiation of DNA replication.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here we investigated the essential DNA replication initiation protein DnaD in the model organism Bacillus subtilis. A set of DnaD residues required for ssDNA binding was identified, and photo-crosslinking revealed that this ssDNA binding region interacts preferentially with one strand of oriC. Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). Considered with single particle cryo-electron microscopy (cryo-EM) imaging of DnaD, we propose that the location of the DRE within oriC orchestrates strand-specific recruitment of helicase during DNA replication initiation. These findings significantly advance our mechanistic understanding of bidirectional replication from a bacterial chromosome origin.

The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin.,Winterhalter C, Pelliciari S, Stevens D, Fenyk S, Marchand E, Cronin NB, Soultanas P, Costa TRD, Ilangovan A, Murray H Nucleic Acids Res. 2023 May 22;51(9):4322-4340. doi: 10.1093/nar/gkad277. PMID:37093985<ref>PMID:37093985</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ojj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Large Structures]]
[[Category: Costa TRD]]
[[Category: Cronin N]]
[[Category: Ilangovan A]]
[[Category: Murray H]]
[[Category: Pelliciari S]]
[[Category: Winterhalter C]]