View source for 8olp


==Y345F/F347Y Variant of Dye Type Peroxidase Aa (DtpAa) from Streptomyces lividans==
<StructureSection load='8olp' size='340' side='right'caption='[[8olp]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8olp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans_1326 Streptomyces lividans 1326]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OLP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.27&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8olp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8olp OCA], [https://pdbe.org/8olp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8olp RCSB], [https://www.ebi.ac.uk/pdbsum/8olp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8olp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A7U9DT46_STRLI A0A7U9DT46_STRLI] Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the protoporphyrin ring intact.[RuleBase:RU365017]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In heme enzymes, such as members of the dye-decolorising peroxidase (DyP) family, the formation of the highly oxidising catalytic Fe(iv)-oxo intermediates following reaction with hydrogen peroxide can lead to free radical migration (hole hopping) from the heme to form cationic tyrosine and/or tryptophan radicals. These species are highly oxidising ( approximately 1 V vs. NHE) and under certain circumstances can catalyse the oxidation of organic substrates. Factors that govern which specific tyrosine or tryptophan the free radical migrates to in heme enzymes are not well understood, although in the case of tyrosyl radical formation the nearby proximity of a proton acceptor is a recognised facilitating factor. By using an A-type member of the DyP family (DtpAa) as an exemplar, we combine protein engineering, X-ray crystallography, hole-hopping calculations, EPR spectroscopy and kinetic modelling to provide compelling new insights into the control of radical migration pathways following reaction of the heme with hydrogen peroxide. We demonstrate that the presence of a tryptophan/tyrosine dyad motif displaying a T-shaped orientation of aromatic rings on the proximal side of the heme dominates the radical migration landscape in wild-type DtpAa and continues to do so following the rational engineering into DtpAa of a previously identified radical migration pathway in an A-type homolog on the distal side of the heme. Only on disrupting the proximal dyad, through removal of an oxygen atom, does the radical migration pathway then switch to the engineered distal pathway to form the desired tyrosyl radical. Implications for protein design and biocatalysis are discussed.

New insights into controlling radical migration pathways in heme enzymes gained from the study of a dye-decolorising peroxidase.,Lucic M, Wilson MT, Pullin J, Hough MA, Svistunenko DA, Worrall JAR Chem Sci. 2023 Oct 6;14(44):12518-12534. doi: 10.1039/d3sc04453j. eCollection , 2023 Nov 15. PMID:38020392<ref>PMID:38020392</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8olp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces lividans 1326]]
[[Category: Hough MA]]
[[Category: Lucic M]]
[[Category: Worrall JAR]]