View source for 8on2


==NI,FE-CODH -600mV state : 24 h Dioxygen Exposure==
<StructureSection load='8on2' size='340' side='right'caption='[[8on2]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8on2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans_Z-2901 Carboxydothermus hydrogenoformans Z-2901]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ON2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ON2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8on2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8on2 OCA], [https://pdbe.org/8on2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8on2 RCSB], [https://www.ebi.ac.uk/pdbsum/8on2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8on2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/COOS2_CARHZ COOS2_CARHZ] CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH) (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ni,Fe-containing carbon monoxide dehydrogenases (CODHs) catalyze the reversible reduction of carbon dioxide to carbon monoxide. CODHs are found in anaerobic microorganisms and can rapidly lose their activity when exposed to air. What causes the loss of activity is unclear. In this study, we analyzed the time-dependent structural changes induced by the presence of air on the metal centers of CODH-II. We show that inactivation is a multistep process. In a reversible step, the open coordination site on the Ni ion is blocked by a Ni,Fe-bridging mu-sulfido or chlorido ligand. Blocking this open coordination site with a cyanide ligand stabilizes the cluster against O(2) -induced decomposition, indicating that O(2) attacks at the Ni ion. In the subsequent irreversible phase, nickel is lost, the Fe ions rearrange and the sulfido ligands disappear. Our data are consistent with a reversible reductive reactivation mechanism to protect CODHs from transient over-oxidation.

Stepwise O(2) -Induced Rearrangement and Disassembly of the [NiFe(4) (OH)(mu(3) -S)(4) ] Active Site Cluster of CO Dehydrogenase.,Basak Y, Jeoung JH, Domnik L, Dobbek H Angew Chem Int Ed Engl. 2023 Aug 7;62(32):e202305341. doi: , 10.1002/anie.202305341. Epub 2023 Jun 30. PMID:37279092<ref>PMID:37279092</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8on2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Carboxydothermus hydrogenoformans Z-2901]]
[[Category: Large Structures]]
[[Category: Basak Y]]
[[Category: Dobbek H]]
[[Category: Jeoung J-H]]