View source for 8pex


==Rho P167L-ATPgS-Psu complex II==
<StructureSection load='8pex' size='340' side='right'caption='[[8pex]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8pex]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_P4 Enterobacteria phage P4] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PEX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pex OCA], [https://pdbe.org/8pex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pex RCSB], [https://www.ebi.ac.uk/pdbsum/8pex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pex ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RHO_ECOLI RHO_ECOLI] Facilitates transcription termination by a mechanism that involves rho binding to the nascent RNA, activation of rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPAse which utilizes all four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01884]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many bacteriophages modulate host transcription to favor expression of their own genomes. Phage satellite P4 polarity suppression protein, Psu, a building block of the viral capsid, inhibits hexameric transcription termination factor, rho, by presently unknown mechanisms. Our cryogenic electron microscopy structures of rho-Psu complexes show that Psu dimers clamp two inactive, open rho rings and promote their expansion to higher-oligomeric states. ATPase, nucleotide binding and nucleic acid binding studies revealed that Psu hinders rho ring closure and traps nucleotides in their binding pockets on rho. Structure-guided mutagenesis in combination with growth, pull-down, and termination assays further delineated the functional rho-Psu interfaces in vivo. Bioinformatic analyses revealed that Psu is associated with a wide variety of phage defense systems across Enterobacteriaceae, suggesting that Psu may regulate expression of anti-phage genes. Our findings show that modulation of the rho oligomeric state via diverse strategies is a pervasive gene regulatory principle in bacteria.

The Psu protein of phage satellite P4 inhibits transcription termination factor rho by forced hyper-oligomerization.,Gjorgjevikj D, Kumar N, Wang B, Hilal T, Said N, Loll B, Artsimovitch I, Sen R, Wahl MC Nat Commun. 2025 Jan 9;16(1):550. doi: 10.1038/s41467-025-55897-9. PMID:39788982<ref>PMID:39788982</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8pex" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Enterobacteria phage P4]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Gjorgjevikj D]]
[[Category: Hilal T]]
[[Category: Loll B]]
[[Category: Wahl MC]]