View source for 8pru


==Engineered form of T thermophiles AHIR==
<StructureSection load='8pru' size='340' side='right'caption='[[8pru]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8pru]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus Thermus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PRU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pru OCA], [https://pdbe.org/8pru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pru RCSB], [https://www.ebi.ac.uk/pdbsum/8pru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pru ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ILVC_THET2 ILVC_THET2] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.[HAMAP-Rule:MF_00435]
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</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus]]
[[Category: Lewis C]]
[[Category: Powell A]]
[[Category: Roberts M]]
[[Category: Sinclair J]]