View source for 8qdu


==Formation of left-handed helices by C2'-fluorinated nucleic acids under physiological salt conditions==
<StructureSection load='8qdu' size='340' side='right'caption='[[8qdu]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8qdu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QDU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QDU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CFL:4-AMINO-1-(2-DEOXY-2-FLUORO-5-O-PHOSPHONO-BETA-D-ARABINOFURANOSYL)PYRIMIDIN-2(1H)-ONE'>CFL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qdu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qdu OCA], [https://pdbe.org/8qdu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qdu RCSB], [https://www.ebi.ac.uk/pdbsum/8qdu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qdu ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Recent findings in cell biology have rekindled interest in Z-DNA, the left-handed helical form of DNA. We report here that two minimally modified nucleosides, 2'F-araC and 2'F-riboG, induce the formation of the Z-form under low ionic strength. We show that oligomers entirely made of these two nucleosides exclusively produce left-handed duplexes that bind to the Zalpha domain of ADAR1. The effect of the two nucleotides is so dramatic that Z-form duplexes are the only species observed in 10 mM sodium phosphate buffer and neutral pH, and no B-form is observed at any temperature. Hence, in contrast to other studies reporting formation of Z/B-form equilibria by a preference for purine glycosidic angles in syn, our NMR and computational work revealed that sequential 2'F...H2N and intramolecular 3'H...N3' interactions stabilize the left-handed helix. The equilibrium between B- and Z- forms is slow in the 19F NMR time scale (&gt;/=ms), and each conformation exhibited unprecedented chemical shift differences in the 19F signals. This observation led to a reliable estimation of the relative population of B and Z species and enabled us to monitor B-Z transitions under different conditions. The unique features of 2'F-modified DNA should thus be a valuable addition to existing techniques for specific detection of new Z-binding proteins and ligands.

Formation of left-handed helices by C2'-fluorinated nucleic acids under physiological salt conditions.,El-Khoury R, Cabrero C, Movilla S, Kaur H, Friedland D, Dominguez A, Thorpe JD, Roman M, Orozco M, Gonzalez C, Damha MJ Nucleic Acids Res. 2024 Jul 22;52(13):7414-7428. doi: 10.1093/nar/gkae508. PMID:38874502<ref>PMID:38874502</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8qdu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Cabrero C]]
[[Category: Damha MJ]]
[[Category: El-Khoury R]]
[[Category: Gonzalez C]]
[[Category: Movilla S]]
[[Category: Orozco M]]
[[Category: Roman M]]
[[Category: Thorpe JD]]