View source for 8qll


==Crystal structure of rat glutathione transferase Omega 1 bound to glutathione==
<StructureSection load='8qll' size='340' side='right'caption='[[8qll]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8qll]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QLL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qll OCA], [https://pdbe.org/8qll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qll RCSB], [https://www.ebi.ac.uk/pdbsum/8qll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qll ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GSTO1_RAT GSTO1_RAT] Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.[UniProtKB:P78417]
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== Publication Abstract from PubMed ==
Glutathione transferases are xenobiotic-metabolizing enzymes with both glutathione-conjugation and ligandin roles. GSTs are present in chemosensory tissues and fluids of the nasal/oral cavities where they protect tissues from exogenous compounds, including food molecules. In the present study, we explored the presence of the omega-class glutathione transferase (GSTO1) in the rat oral cavity. Using immunohistochemistry, GSTO1 expression was found in taste bud cells of the tongue epithelium and buccal cells of the oral epithelium. Buccal and lingual extracts exhibited thiol-transferase activity (4.9 +/- 0.1 and 1.8 +/- 0.1 muM/s/mg, respectively). A slight reduction from 4.9 +/- 0.1 to 4.2 +/- 0.1 muM/s/mg (p &lt; 0.05; Student's t test) was observed in the buccal extract with 100 muM GSTO1-IN-1, a specific inhibitor of GSTO1. RnGSTO1 exhibited the usual activities of omega GSTs, i.e., thiol-transferase (catalytic efficiency of 8.9 x 10(4) M(-1).s(-1)), and phenacyl-glutathione reductase (catalytic efficiency of 8.9 x 10(5) M(-1).s(-1)) activities, similar to human GSTO1. RnGSTO1 interacts with food phytochemicals, including bitter compounds such as luteolin (K(i) = 3.3 +/- 1.9 muM). Crystal structure analysis suggests that luteolin most probably binds to RnGSTO1 ligandin site. Our results suggest that GSTO1 could interact with food phytochemicals in the oral cavity.

Rattus norvegicus Glutathione Transferase Omega 1 Localization in Oral Tissues and Interactions with Food Phytochemicals.,Poirier N, Menetrier F, Moreno J, Boichot V, Heydel JM, Didierjean C, Canivenc-Lavier MC, Canon F, Neiers F, Schwartz M J Agric Food Chem. 2024 Mar 20;72(11):5887-5897. doi: 10.1021/acs.jafc.4c00483. , Epub 2024 Mar 5. PMID:38441878<ref>PMID:38441878</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8qll" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Neiers F]]
[[Category: Schwartz M]]