View source for 8qnd


==Crystal structure of the ribonucleoside hydrolase C from Lactobacillus reuteri==
<StructureSection load='8qnd' size='340' side='right'caption='[[8qnd]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8qnd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Limosilactobacillus_reuteri Limosilactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QND OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QND FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qnd OCA], [https://pdbe.org/8qnd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qnd RCSB], [https://www.ebi.ac.uk/pdbsum/8qnd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qnd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A5JJT3_LIMRT A5JJT3_LIMRT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ribonucleoside hydrolase C (RihC, EC 3.2.2.1, 3.2.2.2, 3.2.2.3, 3.2.2.7, 3.2.2.8) belongs to the family of ribonucleoside hydrolases Rih and catalyzes the cleavage of ribonucleosides to nitrogenous bases and ribose. RihC is one of the enzymes that are synthesized by lactobacilli in response to the presence of Klebsiella. To characterize this protein from Limosilactobacillus reuteri LR1, we cloned and expressed it. The activity of the enzyme was studied towards a wide range of substrates, including ribonucleosides, deoxyribonucleosides as well as an arabinoside. It was shown that the enzyme is active only with ribonucleosides and arabinoside, with the best substrate being uridine. The thermal stability of this enzyme was studied, and its crystal structure was obtained, which demonstrated the tetrameric architecture of the enzyme and allowed to shed light on a correlation between its structure and enzymatic activity. Comprehensive comparisons of all known RihC structures, both existing crystal structures and computed model structures from various species, were made, allowing for the identification of structural motifs important for enzyme functioning.

Structure-Functional Examination of Novel Ribonucleoside Hydrolase C (RihC) from Limosilactobacillus reuteri LR1.,Shaposhnikov LA, Chikurova NY, Atroshenko DL, Savin SS, Kleymenov SY, Chernobrovkina AV, Pometun EV, Minyaev ME, Matyuta IO, Hushpulian DM, Boyko KM, Tishkov VI, Pometun AA Int J Mol Sci. 2023 Dec 30;25(1):538. doi: 10.3390/ijms25010538. PMID:38203708<ref>PMID:38203708</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8qnd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Limosilactobacillus reuteri]]
[[Category: Boyko KM]]
[[Category: Matyuta IO]]
[[Category: Minyaev ME]]
[[Category: Pometun AA]]
[[Category: Popov VO]]
[[Category: Shaposhnikov LA]]
[[Category: Tishkov VI]]