View source for 8qpp


==Bacillus subtilis MutS2-collided disome complex (stalled 70S)==
<StructureSection load='8qpp' size='340' side='right'caption='[[8qpp]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8qpp]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QPP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qpp OCA], [https://pdbe.org/8qpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qpp RCSB], [https://www.ebi.ac.uk/pdbsum/8qpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qpp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3A5I502_BACIU A0A3A5I502_BACIU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli, these pathways are triggered by ribosome collisions through the recruitment of SmrB, a nuclease that cleaves the mRNA. In B. subtilis, the related protein MutS2 was recently implicated in ribosome rescue. Here we show that MutS2 is recruited to collisions by its SMR and KOW domains, and we reveal the interaction of these domains with collided ribosomes by cryo-EM. Using a combination of in vivo and in vitro approaches, we show that MutS2 uses its ABC ATPase activity to split ribosomes, targeting the nascent peptide for degradation through the ribosome quality control pathway. However, unlike SmrB, which cleaves mRNA in E. coli, we see no evidence that MutS2 mediates mRNA cleavage or promotes ribosome rescue by tmRNA. These findings clarify the biochemical and cellular roles of MutS2 in ribosome rescue in B. subtilis and raise questions about how these pathways function differently in diverse bacteria.

B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage.,Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR EMBO J. 2024 Feb;43(4):484-506. doi: 10.1038/s44318-023-00010-3. Epub 2023 Dec , 14. PMID:38177497<ref>PMID:38177497</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8qpp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Aravind L]]
[[Category: Beckmann R]]
[[Category: Berhane R]]
[[Category: Berninghausen O]]
[[Category: Burroughs AM]]
[[Category: Buskirk AR]]
[[Category: Erdenebat C]]
[[Category: Esser H]]
[[Category: Green R]]
[[Category: Mackens-Kiani T]]
[[Category: Park E]]