View source for 8qsx


==Solution NMR structure of the novel adaptor domain TomBN91 from the Tomaymycin non-ribosomal peptide synthetase==
<StructureSection load='8qsx' size='340' side='right'caption='[[8qsx]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8qsx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_regensis Streptomyces regensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QSX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qsx OCA], [https://pdbe.org/8qsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qsx RCSB], [https://www.ebi.ac.uk/pdbsum/8qsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qsx ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
In the quest for new bioactive substances, nonribosomal peptide synthetases (NRPS) provide biodiversity by synthesizing nonproteinaceous peptides with high cellular activity. NRPS machinery consists of multiple modules, each catalyzing a unique series of chemical reactions. Incomplete understanding of the biophysical principles orchestrating these reaction arrays limits the exploitation of NRPSs in synthetic biology. Here, we use nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry to solve the conundrum of how intermodular recognition is coupled with loaded carrier protein specificity in the tomaymycin NRPS. We discover an adaptor domain that directly recruits the loaded carrier protein from the initiation module to the elongation module and reveal its mechanism of action. The adaptor domain of the type found here has specificity rules that could potentially be exploited in the design of engineered NRPS machinery.

The specificity of intermodular recognition in a prototypical nonribosomal peptide synthetase depends on an adaptor domain.,Karanth MN, Kirkpatrick JP, Krausze J, Schmelz S, Scrima A, Carlomagno T Sci Adv. 2024 Jun 21;10(25):eadm9404. doi: 10.1126/sciadv.adm9404. Epub 2024 Jun , 19. PMID:38896613<ref>PMID:38896613</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8qsx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces regensis]]
[[Category: Carlomagno T]]
[[Category: Karanth MN]]
[[Category: Kirkpatrick JP]]