View source for 8qu1


==mt-LSU assembly intermediate in GTPBP8 knock-out cells, state 1==
<StructureSection load='8qu1' size='340' side='right'caption='[[8qu1]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8qu1]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QU1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.74&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qu1 OCA], [https://pdbe.org/8qu1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qu1 RCSB], [https://www.ebi.ac.uk/pdbsum/8qu1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qu1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RM02_HUMAN RM02_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mitochondrial gene expression relies on mitoribosomes to translate mitochondrial mRNAs. The biogenesis of mitoribosomes is an intricate process involving multiple assembly factors. Among these factors, GTP-binding proteins (GTPBPs) play important roles. In bacterial systems, numerous GTPBPs are required for ribosome subunit maturation, with EngB being a GTPBP involved in the ribosomal large subunit assembly. In this study, we focus on exploring the function of GTPBP8, the human homolog of EngB. We find that ablation of GTPBP8 leads to the inhibition of mitochondrial translation, resulting in significant impairment of oxidative phosphorylation. Structural analysis of mitoribosomes from GTPBP8 knock-out cells shows the accumulation of mitoribosomal large subunit assembly intermediates that are incapable of forming functional monosomes. Furthermore, fPAR-CLIP analysis reveals that GTPBP8 is an RNA-binding protein that interacts specifically with the mitochondrial ribosome large subunit 16 S rRNA. Our study highlights the role of GTPBP8 as a component of the mitochondrial gene expression machinery involved in mitochondrial large subunit maturation.

GTPBP8 plays a role in mitoribosome formation in human mitochondria.,Cipullo M, Valentin Gese G, Gopalakrishna S, Krueger A, Lobo V, Pirozhkova MA, Marks J, Palenikova P, Shiriaev D, Liu Y, Misic J, Cai Y, Nguyen MD, Abdelbagi A, Li X, Minczuk M, Hafner M, Benhalevy D, Sarshad AA, Atanassov I, Hallberg BM, Rorbach J Nat Commun. 2024 Jul 5;15(1):5664. doi: 10.1038/s41467-024-50011-x. PMID:38969660<ref>PMID:38969660</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8qu1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Cipullo M]]
[[Category: Hallberg BM]]
[[Category: Rorbach J]]
[[Category: Valentin Gese G]]