View source for 8ran


==Structure of Sen1-RNA complex==
<StructureSection load='8ran' size='340' side='right'caption='[[8ran]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ran]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_2 Human immunodeficiency virus 2] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RAN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.25&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ran FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ran OCA], [https://pdbe.org/8ran PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ran RCSB], [https://www.ebi.ac.uk/pdbsum/8ran PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ran ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SEN1_YEAST SEN1_YEAST] ATP-dependent 5'->3' DNA/RNA helicase required for the expression and maturation of diverse classes of non-protein-coding RNAs like precursor tRNAs, rRNAs and small nuclear (snRNA) and nucleolar (snoRNA) RNAs. Directs RNA polymerase II transcription termination on snoRNAs as well as on several short protein-coding genes. May also play a role in transcription-coupled nucleotide excision repair.<ref>PMID:11565036</ref> <ref>PMID:15121901</ref> <ref>PMID:17157256</ref> <ref>PMID:8943355</ref> <ref>PMID:9365256</ref> <ref>PMID:9819377</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mechanisms underlying the initiation and elongation of RNA polymerase II (Pol II) transcription are well-studied, whereas termination remains poorly understood. Here we analyze the mechanism of polyadenylation-independent Pol II termination mediated by the yeast Sen1 helicase. Cryo-electron microscopy structures of two pretermination intermediates show that Sen1 binds to Pol II and uses its adenosine triphosphatase activity to pull on exiting RNA in the 5' direction. This is predicted to push Pol II forward, induce an unstable hypertranslocated state and destabilize the transcription bubble, thereby facilitating termination. This mechanism of transcription termination may be widely used because it is conceptually conserved in the bacterial transcription system.

Mechanism of polyadenylation-independent RNA polymerase II termination.,Rengachari S, Hainthaler T, Oberthuer C, Lidschreiber M, Cramer P Nat Struct Mol Biol. 2024 Oct 18. doi: 10.1038/s41594-024-01409-0. PMID:39424994<ref>PMID:39424994</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ran" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Human immunodeficiency virus 2]]
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Cramer P]]
[[Category: Lidscreiber M]]
[[Category: Rengachari S]]