View source for 8rc3


==DNA bound type IV-A1 CRISPR effector complex from P. oleovorans==
<StructureSection load='8rc3' size='340' side='right'caption='[[8rc3]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rc3]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_oleovorans Pseudomonas oleovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RC3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rc3 OCA], [https://pdbe.org/8rc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rc3 RCSB], [https://www.ebi.ac.uk/pdbsum/8rc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rc3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A379PIR9_PSEOL A0A379PIR9_PSEOL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulation and plasmid competition. To understand how the type IV-A system associated effector complex achieves this interference, we determine cryo-EM structures of two evolutionarily distinct type IV-A complexes (types IV-A1 and IV-A3) bound to cognate DNA-targets in the presence and absence of the type IV-A signature DinG effector helicase. The structures reveal how the effector complexes recognize the protospacer adjacent motif and target-strand DNA to form an R-loop structure. Additionally, we reveal differences between types IV-A1 and IV-A3 in DNA interactions and structural motifs that allow for in trans recruitment of DinG. Our study provides a detailed view of type IV-A mediated DNA-interference and presents a structural foundation for engineering type IV-A-based genome editing tools.

Structural variation of types IV-A1- and IV-A3-mediated CRISPR interference.,Cepaite R, Klein N, Miksys A, Camara-Wilpert S, Ragozius V, Benz F, Skorupskaite A, Becker H, Zvejyte G, Steube N, Hochberg GKA, Randau L, Pinilla-Redondo R, Malinauskaite L, Pausch P Nat Commun. 2024 Oct 29;15(1):9306. doi: 10.1038/s41467-024-53778-1. PMID:39468082<ref>PMID:39468082</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8rc3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas oleovorans]]
[[Category: Cepaite R]]
[[Category: Malinauskaite L]]
[[Category: Miksys A]]
[[Category: Pausch P]]